概述

产品描述	Rabbit Polyclonal antibody recognizes ATP5F1
反应物种	Hu, Ms
应用	ICC/IF, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ATP5F1
抗原物种	Human
抗原	Recombinant protein of Human ATP5F1
偶联标记	Un-conjugated
別名	PIG47; ATP synthase F(0) complex subunit B1, mitochondrial; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b; ATPase subunit b

应用说明

应用建议	应用 推荐稀释比 ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	HepG2
实际分子量	~ 27 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 11950 Mouse ATP5F1 GeneID: 515 Human ATP5F1 Swiss-port # P24539 Human ATP synthase F(0) complex subunit B1, mitochondrial Swiss-port # Q9CQQ7 Mouse ATP synthase F(0) complex subunit B1, mitochondrial
基因名称	ATP5F1
全名	ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
背景介绍	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008]
生物功能	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. [UniProt]
预测分子量	29 kDa

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