ARG58287
anti-ATP5C1 / ATPG antibody
anti-ATP5C1 / ATPG antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP5C1 / ATPG |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Bov, Mk |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP5C1 / ATPG |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 40-67 (N-terminus) of Human ATP5C1 / ATPG. |
| 偶联标记 | Un-conjugated |
| 別名 | ATP5C; F-ATPase gamma subunit; ATP synthase subunit gamma, mitochondrial; ATP5CL1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HL-60 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P36542 Human ATP synthase subunit gamma, mitochondrial |
|---|---|
| 基因名称 | ATP5C1 |
| 全名 | ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 |
| 背景介绍 | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008] |
| 生物功能 | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProt] |
| 细胞定位 | Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein. [UniProt] |
| 预测分子量 | 33 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58287 anti-ATP5C1 / ATPG antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skeletal muscle stained with ARG58287 anti-ATP5C1 / ATPG antibody.
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ARG58287 anti-ATP5C1 / ATPG antibody WB image
Western blot: 35 µg of HL-60 cell lysate stained with ARG58287 anti-ATP5C1 / ATPG antibody.
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ARG58287 anti-ATP5C1 / ATPG antibody FACS image
Flow Cytometry: ZR-75-1 cells stained with ARG58287 anti-ATP5C1 / ATPG antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
