ARG65744
anti-ATP2B1 / PMCA1 antibody
anti-ATP2B1 / PMCA1 antibody for Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes ATP2B1 / PMCA1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP2B1 / PMCA1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human ATP2B1. (C-KQDGAIENRNKAKAQD) |
| 偶联标记 | Un-conjugated |
| 別名 | PMCA1; PMCA1kb; EC 3.6.3.8; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium pump isoform 1; Plasma membrane calcium-transporting ATPase 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20020 Human Plasma membrane calcium-transporting ATPase 1 |
|---|---|
| 基因名称 | ATP2B1 |
| 全名 | ATPase, Ca++ transporting, plasma membrane 1 |
| 背景介绍 | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. [UniProt] |
| 预测分子量 | 135 kDa (NP_001673.2) |
检测图片 (1) Click the Picture to Zoom In
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ARG65744 anti-ATP2B1 / PMCA1 antibody WB image
Western blot: 35 µg of HeLa lysate stained with ARG65744 anti-ATP2B1 / PMCA1 antibody at 0.03 µg/ml dilution (1 hour incubation).
