ARG58285
anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody
anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP2A1 / SERCA1 ATPase (isoform b) |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Rb |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP2A1 / SERCA1 ATPase (isoform b) |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 974-1001 of Human ATP2A1 / SERCA1 ATPase (isoform b). |
| 偶联标记 | Un-conjugated |
| 別名 | SERCA1; ATP2A; SR Ca; Endoplasmic reticulum class 1/2 Ca; EC 3.6.3.8; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; 2+ |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HT1080 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O14983 Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 |
|---|---|
| 基因名称 | ATP2A1 |
| 全名 | ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 |
| 背景介绍 | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013] |
| 生物功能 | Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 110 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58285 anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skeletal muscle stained with ARG58285 anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody.
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ARG58285 anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody WB image
Western blot: 35 µg of HT1080 cell lysate stained with ARG58285 anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody at 1:1000 dilution.
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ARG58285 anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody FACS image
Flow Cytometry: K562 cells stained with ARG58285 anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
