ARG42539
anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody
anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Dog,Human,Monkey,Mouse,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes ATP1A1 / Na+ K+ ATPase alpha 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | IHC-Fr, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP1A1 / Na+ K+ ATPase alpha 1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant peptide within aa. 100 to the N-terminus of Human ATP1A1 / Na+ K+ ATPase alpha 1. |
| 偶联标记 | Un-conjugated |
| 別名 | Sodium pump subunit alpha-1; Na; +; Sodium/potassium-transporting ATPase subunit alpha-1; EC 3.6.3.9 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | LS174T | ||||||||
| 实际分子量 | ~ 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATP1A1 |
| 全名 | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
| 背景介绍 | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| 生物功能 | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. [UniProt] |
| 细胞定位 | Cell membrane, sarcolemma; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| 预测分子量 | 113 kDa |
| 翻译后修饰 | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity). [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody IHC-P image
Immunohistochemistry: Human skin stained with ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody at 1:500 dilution.
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ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody WB image
Western blot: 50 µg of LS174T cell lysate stained with ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody at 1:1000 dilution.
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ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody IHC-P image
Immunohistochemistry: Rat eye stained with ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody at 1:500 dilution.
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ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG42539 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody at 1:500 dilution.
