ARG58251
anti-ATP Citrate Lyase phospho (Ser455) antibody
anti-ATP Citrate Lyase phospho (Ser455) antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP Citrate Lyase phospho (Ser455) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP Citrate Lyase |
| 抗原物种 | Human |
| 抗原 | Phospho specific peptide around Ser455 of Human ATP Citrate Lyase (NP_001087.2). |
| 偶联标记 | Un-conjugated |
| 別名 | ACL; ATP-citrate synthase; Citrate cleavage enzyme; CLATP; EC 2.3.3.8; pro-S-; ATP-citrate; ATPCL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | NIH/3T3 + insulin | ||||||||
| 实际分子量 | 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACLY |
| 全名 | ATP citrate lyase |
| 背景介绍 | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014] |
| 生物功能 | ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 121 kDa |
| 翻译后修饰 | ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable). [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody WB image
Western blot: 25 µg of NIH/3T3 cells treated by Insulin (100 nM) for 10 min after serum-starvation overnight. The blot was stained with ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody at 1:2000 dilution.
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ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse pancreas tissue stained with ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody at 1:100 dilution.
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ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody IP image
Immunoprecipitation: NIH/3T3 cells were treated by Insulin (100 nM) at 37°C for 10 minutes after serum-starvation overnight. 200 µg extracts of NIH/3T3 cells were immunoprecipitated and stained with ARG58251 anti-ATP Citrate Lyase phospho (Ser455) antibody at 1:1000 dilution.
