ARG54041
anti-ATP Citrate Lyase antibody (C-term)
anti-ATP Citrate Lyase antibody (C-term) for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Monkey
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes ACLY |
|---|---|
| 反应物种 | Hu, Ms, Mk |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2a |
| 靶点名称 | ATP Citrate Lyase |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | ACL; ATP-citrate synthase; Citrate cleavage enzyme; CLATP; EC 2.3.3.8; pro-S-; ATP-citrate; ATPCL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACLY |
| 全名 | ATP citrate lyase |
| 背景介绍 | ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. |
| 生物功能 | ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 121 kDa |
| 翻译后修饰 | ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable). |
检测图片 (3) Click the Picture to Zoom In
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ARG54041 anti-ATP Citrate Lyase antibody (C-term) ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54041 anti-ATP Citrate Lyase antibody (C-term) at 1:150 dilution.
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ARG54041 anti-ATP Citrate Lyase antibody (C-term) FACS image
Flow Cytometry: HeLa cells stained with ARG54041 anti-ATP Citrate Lyase antibody (C-term) at 1:100 dilution (right) or isotype control antibody (left), followed by incubation with FITC labelled secondary antibody.
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ARG54041 anti-ATP Citrate Lyase antibody (C-term) WB image
Western blot: K562 cell lysate stained with ARG54041 anti-ATP Citrate Lyase antibody (C-term) at 1:1000 dilution.
