ARG42696
anti-ATP Citrate Lyase antibody [5I2]
anti-ATP Citrate Lyase antibody [5I2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [5I2] recognizes ATP Citrate Lyase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5I2 |
| 同位型 | IgG2b |
| 靶点名称 | ATP Citrate Lyase |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to M1-I180 of Human ATP Citrate Lyase. |
| 偶联标记 | Un-conjugated |
| 別名 | ACL; ATP-citrate synthase; Citrate cleavage enzyme; CLATP; EC 2.3.3.8; pro-S-; ATP-citrate; ATPCL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | ~ 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACLY |
| 全名 | ATP citrate lyase |
| 背景介绍 | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014] |
| 生物功能 | Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 121 kDa |
| 翻译后修饰 | ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable). [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] ICC/IF image
Immunofluorescence: MCF7 cells were blocked with 10% goat serum and then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreatic cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] WB image
Western blot: 50 µg of samples under reducing condition. Human placenta, U-87MG, HEK293, Caco-2, HL-60, Raji, THP-1 and PANC-1 whole cell lysates stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse pancreas tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat pancreas tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
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ARG42696 anti-ATP Citrate Lyase antibody [5I2] WB image
Western blot: 50 µg of samples under reducing condition. Rat lung, Rat testis, Rat kidney, Rat brain, Mouse lung, Mouse testis, Mouse kidney and Mouse brain lysates stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 0.5 µg/ml dilution, overnight at 4°C.
