ARG54904
anti-ATG4A antibody
anti-ATG4A antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
参考文献2
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG4A |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG4A |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human ATG4A (NP_443168.2) |
| 偶联标记 | Un-conjugated |
| 別名 | Cysteine protease ATG4A; Autophagin-2; AUT-like 2 cysteine endopeptidase; APG4A; Autophagy-related cysteine endopeptidase 2; EC 3.4.22.-; AUTL2; Autophagy-related protein 4 homolog A; hAPG4A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | BT474 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG4A |
| 全名 | autophagy related 4A, cysteine peptidase |
| 背景介绍 | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| 预测分子量 | 45 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54904 anti-ATG4A antibody WB image
Western blot: BT474 cell lysate stained with ARG54904 anti-ATG4A antibody.
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ARG54904 anti-ATG4A antibody IP image
Immunoprecipitation: K562 cell lysate was immunoprecipitated and stained with ARG54904 anti-ATG4A antibody.
文献引用
Reduced systemic autophagy by simultaneous loss of ATG4B, ATG4C and ATG4D leads to accelerated aging in mice
WB / Mouse
Analysis of ATG4C function in vivo
WB / Human
