ARG54816
anti-ATG3 antibody [1377CT239.6.1.12]
anti-ATG3 antibody [1377CT239.6.1.12] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes ATG3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1377CT239.6.1.12 |
| 同位型 | IgG1, kappa |
| 靶点名称 | ATG3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human ATG3. |
| 偶联标记 | Un-conjugated |
| 別名 | Ubiquitin-like-conjugating enzyme ATG3; EC 6.3.2.-; APG3-LIKE; Protein PC3-96; APG3; Autophagy-related protein 3; PC3-96; APG3L; hApg3; APG3-like |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse liver |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9CPX6 Mouse Ubiquitin-like-conjugating enzyme ATG3 Swiss-port # Q9NT62 Human Ubiquitin-like-conjugating enzyme ATG3 |
|---|---|
| 基因名称 | ATG3 |
| 全名 | autophagy related 3 |
| 背景介绍 | This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| 生物功能 | E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. [UniProt] |
| 细胞定位 | Cytoplasm. |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis. |
检测图片 (2) Click the Picture to Zoom In
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ARG54816 anti-ATG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney section stained with ARG54816 anti-ATG3 antibody at 1:25 dilution.
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ARG54816 anti-ATG3 antibody WB image
Western blot: 20 µg of Mouse liver lysate stained with ARG54816 anti-ATG3 antibody at 1:1000 dilution.
