ARG59071
anti-ATG14 / ATG14L antibody
anti-ATG14 / ATG14L antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG14 / ATG14L |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Hm |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG14 / ATG14L |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 70-101 of Human ATG14 / ATG14L (RDRERFIDKKERLSRLKSKQEEFQKEVLKAME). |
| 偶联标记 | Un-conjugated |
| 別名 | BARKOR; Autophagy-related protein 14-like protein; Beclin 1-associated autophagy-related key regulator; Barkor; KIAA0831; Atg14L; ATG14L |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 57 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # D4A4K3 Rat Beclin 1-associated autophagy-related key regulator Swiss-port # Q6ZNE5 Human Beclin 1-associated autophagy-related key regulator |
|---|---|
| 基因名称 | ATG14 |
| 全名 | autophagy related 14 |
| 生物功能 | Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein. [UniProt] |
| 预测分子量 | 55 kDa |
检测图片 (6) Click the Picture to Zoom In
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ARG59071 anti-ATG14 / ATG14L antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59071 anti-ATG14 / ATG14L antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59071 anti-ATG14 / ATG14L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59071 anti-ATG14 / ATG14L antibody at 1 µg/ml, overnight at 4°C.
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ARG59071 anti-ATG14 / ATG14L antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain and HeLa whole cell lysates stained with ARG59071 anti-ATG14 / ATG14L antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59071 anti-ATG14 / ATG14L antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59071 anti-ATG14 / ATG14L antibody at 5 μg/mL.
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ARG59071 anti-ATG14 / ATG14L antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG59071 anti-ATG14 / ATG14L antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59071 anti-ATG14 / ATG14L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59071 anti-ATG14 / ATG14L antibody at 1 µg/ml, overnight at 4°C.
