ARG63137
anti-ATF7 antibody
anti-ATF7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ATF7 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATF7 |
| 抗原物种 | Human |
| 抗原 | C-HVIMTPQSQSAGR |
| 偶联标记 | Un-conjugated |
| 別名 | cAMP-dependent transcription factor ATF-7; Transcription factor ATF-A; ATFA; Activating transcription factor 7; Cyclic AMP-dependent transcription factor ATF-7 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17544 Human Cyclic AMP-dependent transcription factor ATF-7 |
|---|---|
| 基因名称 | ATF7 |
| 全名 | activating transcription factor 7 |
| 生物功能 | Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter. Isoform 5/ATF-4 acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity. Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase. |
检测图片 (2) Click the Picture to Zoom In
-
ARG63137 anti-ATF7 antibody WB image
Western Blot: HeLa lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63137 anti-ATF7 antibody at 0.5 µg/ml dilution.
-
ARG63137 anti-ATF7 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63137 anti-ATF7 antibody at 3.8 µg/ml dilution followed by AP-staining.
