ARG41990
anti-ATF5 antibody
anti-ATF5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATF5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATF5 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human ATF5. |
| 偶联标记 | Un-conjugated |
| 別名 | HMFN0395; cAMP-dependent transcription factor ATF-5; Cyclic AMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx; ATFX |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Jurkat | ||||||||||
| 实际分子量 | ~ 32 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATF5 |
| 全名 | activating transcription factor 5 |
| 生物功能 | Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, translocates from the centrosome to the midbody. [UniProt] |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | Ubiquitinated by CDC34 and UBE2B in order to be degraded by the proteasome. Cisplatin inhibits ubiquitination and proteasome-mediated degradation by inhibiting the interaction with CDC34 (PubMed:18458088). Ubiquitination and degradation by the proteasome are inhibited by NLK in a kinase-independent manner (PubMed:25512613). Phosphorylated by NLK, probably at Ser-92, Thr-94, Ser-126 and Ser-190. Acetylated at Lys-29 by EP300, the acetylation enhances the interaction with CEBPB, DNA-binding and transactivation activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41990 anti-ATF5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue stained with ARG41990 anti-ATF5 antibody.
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ARG41990 anti-ATF5 antibody WB image
Western blot: Jurkat cell lysate stained with ARG41990 anti-ATF5 antibody.
