ARG51557
anti-ATF4 phospho (Ser245) antibody
anti-ATF4 phospho (Ser245) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATF4 phospho (Ser245) |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATF4 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 245 (N-R-S(p)-L-P) derived from Human ATF4. |
| 偶联标记 | Un-conjugated |
| 別名 | DNA-binding protein TAXREB67; TAXREB67; Tax-responsive enhancer element-binding protein 67; cAMP-dependent transcription factor ATF-4; Cyclic AMP-dependent transcription factor ATF-4; Activating transcription factor 4; CREB2; CREB-2; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; TXREB; TaxREB67 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P18848 Human Cyclic AMP-dependent transcription factor ATF-4 |
|---|---|
| 基因名称 | ATF4 |
| 全名 | activating transcription factor 4 |
| 背景介绍 | ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. |
| 生物功能 | Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4. Phosphorylated by NEK6 (By similarity). Phosphorylated on the betaTrCP degron motif at Ser-219, followed by phosphorylation at Thr-213, Ser-224, Ser-231, Ser-235 and Ser-248, promoting interaction with BTRC and ubiquitination. Phosphorylation is promoted by mTORC1 (By similarity). Phosphorylated by NEK6. |
检测图片 (2) Click the Picture to Zoom In
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ARG51557 anti-ATF4 phospho (Ser245) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with UV stained with ARG51557 anti-ATF4 phospho (Ser245) antibody.
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ARG51557 anti-ATF4 phospho (Ser245) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51557 anti-ATF4 phospho (Ser245) antibody (left) or the same antibody preincubated with blocking peptide (right).
