ARG52232
anti-ATF2 phospho (Thr52) antibody
anti-ATF2 phospho (Thr52) antibody for Western blot and Human
Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATF2 phospho (Thr52) |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATF2 |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding THr52 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.3.1.48; Histone acetyltransferase ATF2; Activating transcription factor 2; cAMP-dependent transcription factor ATF-2; Cyclic AMP-dependent transcription factor ATF-2; CREB2; HB16; CREB-2; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; CRE-BP1; cAMP response element-binding protein CRE-BP1; TREB7 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human UACC903 melanoma cells |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P15336 Human Cyclic AMP-dependent transcription factor ATF-2 |
|---|---|
| 基因名称 | ATF2 |
| 全名 | activating transcription factor 2 |
| 背景介绍 | The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKCε regulates this translocation (Lau et al., 2012). |
| 研究领域 | Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances its histone acetyltransferase (HAT) activity. |
