概述

产品描述	Rabbit Polyclonal antibody recognizes ATF1
反应物种	Hu
应用	ICC/IF, IP, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ATF1
抗原物种	Human
抗原	Synthetic peptide derived from Human ATF1.
偶联标记	Un-conjugated
別名	Cyclic AMP-dependent transcription factor ATF-1; TREB36; Activating transcription factor 1; EWS-ATF1; cAMP-dependent transcription factor ATF-1; Protein TREB36; FUS/ATF-1

应用说明

应用建议	应用 推荐稀释比 ICC/IF 1:50 - 1:200 IP 1:50 WB 1:1000 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 466 Human ATF1 Swiss-port # P18846 Human Cyclic AMP-dependent transcription factor ATF-1
基因名称	ATF1
全名	activating transcription factor 1
背景介绍	This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]
生物功能	This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. [UniProt]
细胞定位	Nucleus. [UniProt]
预测分子量	29 kDa
翻译后修饰	Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. [UniProt]

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