ARG45756

anti-ASIC2 antibody

anti-ASIC2 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes ASIC2
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 ASIC2
抗原物种 Human
抗原 Recombinant protein containing to human ASIC2.
偶联标记 Un-conjugated
別名 acid sensing (proton gated) ion channel 2; ACCN1; ACCN; BNC1; Amiloride-sensitive cation channel 1, neuronal; MDEG; Mammalian degenerin homolog; Amiloride-sensitive cation channel neuronal 1; BNaC1; ASIC2; Amiloride-sensitive brain sodium channel; Acid-sensing ion channel 2; hBNaC1; ASIC2a; Brain sodium channel 1

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF2 μg/ml
WB0.1-0.25 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 58 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11418 Mouse ASIC2

GeneID: 25364 Rat ASIC2

GeneID: 40 Human ASIC2

基因名称 ASIC2
全名 acid sensing (proton gated) ion channel 2
背景介绍 This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]
生物功能 Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. [UniProt]
细胞定位 Cell membrane; Multi-pass membrane protein. Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. [UniProt]
预测分子量 58 kDa
翻译后修饰 Disulfide bond; Glycoprotein; Phosphoprotein. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45756 anti-ASIC2 antibody ICC/IF image

    Immunofluorescence: A431 stained with ARG45756 anti-ASIC2 antibody at 5 μg/ml dilution.

  • ARG45756 anti-ASIC2 antibody WB image

    Western blot: Human placenta stained with ARG45756 anti-ASIC2 antibody at 0.5 μg/ml dilution.

  • ARG45756 anti-ASIC2 antibody FACS image

    Flow Cytometry: A549 stained with ARG45756 anti-ASIC2 antibody at 1 µg/10^6 cells dilution.

  • ARG45756 anti-ASIC2 antibody WB image

    Western blot: Rat brain stained with ARG45756 anti-ASIC2 antibody at 0.5 μg/ml dilution.

  • ARG45756 anti-ASIC2 antibody WB image

    Western blot: Mouse brain stained with ARG45756 anti-ASIC2 antibody at 0.5 μg/ml dilution.