ARG43170
anti-ASH2L antibody
anti-ASH2L antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ASH2L |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ASH2L |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human ASH2L. |
| 偶联标记 | Un-conjugated |
| 別名 | ASH2-like protein; ASH2L1; ASH2L2; Set1/Ash2 histone methyltransferase complex subunit ASH2; Bre2; ASH2 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | K562 | ||||||||
| 实际分子量 | 69, 80 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q91X20 Mouse Set1/Ash2 histone methyltransferase complex subunit ASH2 Swiss-port # Q9UBL3 Human Set1/Ash2 histone methyltransferase complex subunit ASH2 |
|---|---|
| 基因名称 | ASH2L |
| 全名 | ash2 (absent, small, or homeotic)-like (Drosophila) |
| 生物功能 | Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG43170 anti-ASH2L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kideny tissue stained with ARG43170 anti-ASH2L antibody.
-
ARG43170 anti-ASH2L antibody WB image
Western blot: K562 cell lysate stained with ARG43170 anti-ASH2L antibody.
