ARG42092
anti-ASCL3 antibody
anti-ASCL3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Goat Polyclonal antibody recognizes ASCL3 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ASCL3 |
| 抗原物种 | Mouse |
| 抗原 | Synthetic peptide around the internal region of Mouse Ascl3. (C-HLPEDYLEKRLSK) (NP_064435.1) |
| 偶联标记 | Un-conjugated |
| 別名 | HASH3; SGN1; Achaete-scute homolog 3; bHLH transcriptional regulator Sgn-1; hASH3; ASH-3; bHLHa42; Class A basic helix-loop-helix protein 42 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Mouse liver | ||||||
| 实际分子量 | ~ 22 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ASCL3 |
| 全名 | achaete-scute family bHLH transcription factor 3 |
| 背景介绍 | Basic helix-loop-helix transcription factors, such as ASCL3, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Transcriptional repressor. Inhibits myogenesis (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 20 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42092 anti-ASCL3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42092 anti-ASCL3 antibody at 3.75 µg/ml dilution.
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ARG42092 anti-ASCL3 antibody WB image
Western blot: 35 µg of Mouse liver lysate (in RIPA buffer) stained with ARG42092 anti-ASCL3 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
