ARG40258
anti-ASC1 antibody
anti-ASC1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ASC1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ASC1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 282-581 of Human ASC1 (NP_057297.2). |
| 偶联标记 | Un-conjugated |
| 別名 | TRIP-4; ZC2HC5; TR-interacting protein 4; HsT17391; ASC-1; ASC1; Activating signal cointegrator 1; Thyroid receptor-interacting protein 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A549, Mouse brain and Rat brain | ||||||||
| 实际分子量 | 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TRIP4 |
| 全名 | thyroid hormone receptor interactor 4 |
| 生物功能 | Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation. Also involved in androgen receptor transactivation (By similarity). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic under conditions of serum deprivation (PubMed:10454579). Colocalizes with NEK6 in the centrosome (PubMed:20873783). [UniProt] |
| 预测分子量 | 66 kDa |
| 翻译后修饰 | Phosphorylated by NEK6. Polyufmylated by the UFM1-conjugating system composed of the enzymes UBA5, UFC1 and UFL1. Deufmylated by the protease UFSP2. Ufmylation of TRIP4 is promoted by ligand-bound nuclear receptors that compete with UFSP2 for interaction with TRIP4. Nuclear receptors-induced ufmylation promotes the recruitment of additional transcriptional coactivators like EP300 and NCOA1 and therefore the assembly of a coactivator complex facilitating nuclear receptor-mediated transcription. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG40258 anti-ASC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG40258 anti-ASC1 antibody at 1:100 dilution.
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ARG40258 anti-ASC1 antibody WB image
Western blot: 25 µg of Mouse brain and A549 cell lysates stained with ARG40258 anti-ASC1 antibody at 1:1000 dilution.
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ARG40258 anti-ASC1 antibody IP image
Immunoprecipitation: 200 µg extracts of A549 cells were immunoprecipitated and stained with ARG40258 anti-ASC1 antibody at 1:1000 dilution.
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ARG40258 anti-ASC1 antibody WB image
Western blot: 25 µg of Rat brain lysate stained with ARG40258 anti-ASC1 antibody at 1:1000 dilution.
