概述

产品描述	Rabbit Polyclonal antibody recognizes ASAH2
反应物种	Hu, Ms, Rat
应用	WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ASAH2
抗原物种	Human
抗原	Recombinant Protein of Human ASAH2.
偶联标记	Un-conjugated
別名	hCD; EC 3.5.1.23; HNAC1; LCDase; Acylsphingosine deacylase 2; Neutral ceramidase; NCDase; Non-lysosomal ceramidase; BCDase; N-CDase; N-acylsphingosine amidohydrolase 2

应用说明

应用建议	应用 推荐稀释比 WB 1:500 - 2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	Mouse kidney

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 114104 Rat ASAH2 GeneID: 54447 Mouse ASAH2 GeneID: 56624 Human ASAH2 Swiss-port # Q91XT9 Rat Neutral ceramidase Swiss-port # Q9JHE3 Mouse Neutral ceramidase Swiss-port # Q9NR71 Human Neutral ceramidase More
基因名称	ASAH2
全名	N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
背景介绍	Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
生物功能	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. [UniProt]
预测分子量	86 kDa
翻译后修饰	N-glycosylated. Required for enzyme activity (By similarity). O-glycosylated. Required to retain it as a type II membrane protein at the cell surface. Phosphorylated. May prevent ubiquitination and subsequent degradation (By similarity). Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid (By similarity).

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