ARG64136
anti-AS160 / TBC1D4 antibody
anti-AS160 / TBC1D4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes AS160 / TBC1D4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AS160 / TBC1D4 |
| 抗原物种 | Human |
| 抗原 | C-DDPEKIEERKKSK |
| 偶联标记 | Un-conjugated |
| 別名 | AS160; TBC1 domain family member 4; Akt substrate of 160 kDa; NIDDM5 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TBC1D4 |
| 全名 | TBC1 domain family, member 4 |
| 背景介绍 | This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
| 生物功能 | May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 147 kDa |
| 翻译后修饰 | Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. |
检测图片 (2) Click the Picture to Zoom In
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ARG64136 anti-AS160 / TBC1D4 antibody WB image
Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG64136 anti-AS160 / TBC1D4 antibody at 0.1 µg/ml dilution.
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ARG64136 anti-AS160 / TBC1D4 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Skeletal Muscle. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64136 anti-AS160 / TBC1D4 antibody at 3.8 µg/ml dilution followed by AP-staining.
