ARG64841
anti-ARNTL / BMAL1 antibody
anti-ARNTL / BMAL1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ARNTL / BMAL1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_001025444.1; NP_001025443.1). Reported variants represent identical protein (NP_001025443.1; NP_001169.3). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARNTL / BMAL1 |
| 抗原物种 | Human |
| 抗原 | REKITTNCYKFKIKD |
| 偶联标记 | Un-conjugated |
| 別名 | BMAL1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; bHLH-PAS protein JAP3; PAS domain-containing protein 3; Aryl hydrocarbon receptor nuclear translocator-like protein 1; MOP3; JAP3; Class E basic helix-loop-helix protein 5; bHLHe5; Member of PAS protein 3; PASD3; BMAL1c; TIC |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00327 Human Aryl hydrocarbon receptor nuclear translocator-like protein 1 |
|---|---|
| 背景介绍 | The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Ubiquitinated, leading to its proteasomal degradation. O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2. Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability. Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry (By similarity). Dephosphorylation at Ser-78 is important for dimerization with CLOCK and transcriptional activity (PubMed:23229515). Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer. |
检测图片 (1) Click the Picture to Zoom In
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ARG64841 anti-ARNTL / BMAL1 antibody WB image
Western blot: Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer) stained with ARG64841 anti-ARNTL / BMAL1 antibody at 0.3 µg/ml dilution.
