ARG42377
anti-ARHGEF4 antibody [ARHGEF-08] (PE)
anti-ARHGEF4 antibody [ARHGEF-08] (PE) for Flow cytometry and Human
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [ARHGEF-08] recognizes ARHGEF4 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ARHGEF-08 |
| 同位型 | IgG1 |
| 靶点名称 | ARHGEF4 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment corresponding to aa. 143-271 of Human ARHGEF4. |
| 偶联标记 | PE |
| 別名 | STM6; Rho guanine nucleotide exchange factor 4; Asef; GEF4; APC-stimulated guanine nucleotide exchange factor 1; Asef1; ASEF; ASEF1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NR80 Human Rho guanine nucleotide exchange factor 4 |
|---|---|
| 基因名称 | ARHGEF4 |
| 全名 | Rho guanine nucleotide exchange factor (GEF) 4 |
| 背景介绍 | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013] |
| 生物功能 | Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. [UniProt] |
| 细胞定位 | Isoform 3: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with membrane ruffles. [UniProt] |
| 预测分子量 | 79 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42377 anti-ARHGEF4 antibody [ARHGEF-08] (PE) FACS image
Flow Cytometry: Transfected HEK-293 cells stained with ARG42377 anti-ARHGEF4 antibody [ARHGEF-08] (PE) at 1 µg/ml dilution (blue) and ARG65337 Mouse IgG1 Kappa Isotype Control antibody [MOPC-21] (PE) at 1 µg/ml dilution (orange).
