ARG59029

anti-ARHGEF1 antibody

Datasheet

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Rabbit Polyclonal antibody recognizes ARHGEF1
反应物种	Hu, Ms, Rat
预测物种	Hm
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ARHGEF1
抗原物种	Human
抗原	Synthetic peptide corresponding to aa. 41-71 of Human ARHGEF1 (EQNSQFQSLEQVKRRPAHLMALLQHVALQFE).
偶联标记	Un-conjugated
別名	p115RhoGEF; LSC; SUB1.5; Sub1.5; LBCL2; Rho guanine nucleotide exchange factor 1; P115-RHOGEF; 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; GEF1

应用说明

应用建议

应用	推荐稀释比
FACS	1:150 - 1:500
ICC/IF	1:200 - 1:1000
IHC-P	0.5 - 1 µg/ml
WB	0.1 - 0.5 µg/ml

应用说明

IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
抗菌剂	0.05% Sodium azide
稳定剂	5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 16801 Mouse ARHGEF1 GeneID: 9138 Human ARHGEF1 Swiss-port # Q61210 Mouse Rho guanine nucleotide exchange factor 1 Swiss-port # Q92888 Human Rho guanine nucleotide exchange factor 1
基因名称	ARHGEF1
全名	Rho guanine nucleotide exchange factor (GEF) 1
背景介绍	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
生物功能	Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. [UniProt]
细胞定位	Cytoplasm. Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation. [UniProt]
预测分子量	102 kDa
翻译后修饰	Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. [UniProt]

检测图片 (5) Click the Picture to Zoom In

ARG59029 anti-ARHGEF1 antibody ICC/IF image

Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG59029 anti-ARHGEF1 antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG59029 anti-ARHGEF1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse lymphaden tissue stained with ARG59029 anti-ARHGEF1 antibody at 1 µg/ml dilution.
ARG59029 anti-ARHGEF1 antibody WB image

Western blot: Rat brain, HeLa and Jurkat lysates stained with ARG59029 anti-ARHGEF1 antibody at 0.5 µg/ml dilution.
ARG59029 anti-ARHGEF1 antibody FACS image

Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59029 anti-ARHGEF1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG59029 anti-ARHGEF1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat lymphaden tissue stained with ARG59029 anti-ARHGEF1 antibody at 1 µg/ml dilution.