ARG58958
anti-ARHGEF1 antibody
anti-ARHGEF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ARHGEF1 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARHGEF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 783-912 of Human ARHGEF1 (NP_004697.2). |
| 偶联标记 | Un-conjugated |
| 別名 | p115RhoGEF; LSC; SUB1.5; Sub1.5; LBCL2; Rho guanine nucleotide exchange factor 1; P115-RHOGEF; 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; GEF1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | 102 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q92888 Human Rho guanine nucleotide exchange factor 1 |
|---|---|
| 基因名称 | ARHGEF1 |
| 全名 | Rho guanine nucleotide exchange factor (GEF) 1 |
| 背景介绍 | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. [UniProt] |
| 细胞定位 | Cytoplasm, Membrane. [UniProt] |
| 预测分子量 | 102 kDa |
| 翻译后修饰 | Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG58958 anti-ARHGEF1 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG58958 anti-ARHGEF1 antibody at 1:3000 dilution.
