ARG56849
anti-ARFBP1 antibody
anti-ARFBP1 antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ARFBP1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARFBP1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (19 aa) within aa. 4250-4300 of Human ARF-BP1. |
| 偶联标记 | Un-conjugated |
| 別名 | HECT, UBA and WWE domain-containing protein 1; HectH9; EC 6.3.2.-; HSPC272; ARF-binding protein 1; Mule; Homologous to E6AP carboxyl terminus homologous protein 9; E3 ubiquitin-protein ligase HUWE1; HECTH9; ARF-BP1; URE-binding protein 1; Ib772; Mcl-1 ubiquitin ligase E3; LASU1; UREB1; Large structure of UREB1; MULE; Upstream regulatory element-binding protein 1; URE-B1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Daudi cell lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HUWE1 |
| 全名 | HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic mental retardation. [provided by RefSeq, Aug 2013] |
| 生物功能 | E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4. [UniProt] |
| 预测分子量 | 482 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation. |
检测图片 (3) Click the Picture to Zoom In
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ARG56849 anti-ARFBP1 antibody ICC/IF image
Immunocytochemistry: Daudi cells stained with ARG56849 anti-ARFBP1 antibody at 5 µg/ml dilution.
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ARG56849 anti-ARFBP1 antibody WB image
Western blot: Daudi cell lysate stained with ARG56849 anti-ARFBP1 antibody at 1 µg/ml dilution.
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ARG56849 anti-ARFBP1 antibody ICC/IF image
Immunofluorescence: Daudi cells stained with ARG56849 anti-ARFBP1 antibody at 20 µg/ml dilution.
