ARG58199
anti-ARF4 antibody
anti-ARF4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Goat Polyclonal antibody recognizes ARF4 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARF4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the internal region of Human ARF4 (NP_001651.1). (C-SEMTDKLGLQSLRN) |
| 偶联标记 | Un-conjugated |
| 別名 | ARF2; ADP-ribosylation factor 4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 19 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ARF4 |
| 全名 | ADP-ribosylation factor 4 |
| 背景介绍 | This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. [UniProt] |
| 预测分子量 | 21 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG58199 anti-ARF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus stained with ARG58199 anti-ARF4 antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
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ARG58199 anti-ARF4 antibody WB image
Western blot: 35 µg of NIH/3T3 and HeLa cell lysates (in RIPA buffer) stained with ARG58199 anti-ARF4 antibody at 0.5 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
