ARG43287
anti-AREG / Amphiregulin antibody
anti-AREG / Amphiregulin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AREG / Amphiregulin |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AREG / Amphiregulin |
| 抗原物种 | Mouse |
| 抗原 | Recombinant protein corresponding to V100-K191 of Mouse AREG / Amphiregulin. |
| 偶联标记 | Un-conjugated |
| 別名 | Amphiregulin; AREGB; CRDGF; SDGF; AR; Colorectum cell-derived growth factor |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Rat brain and Mouse brain | ||||||
| 实际分子量 | ~ 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AREG |
| 全名 | amphiregulin |
| 背景介绍 | The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014] |
| 生物功能 | Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. [UniProt] |
| 细胞定位 | Membrane; Single-pass membrane protein. [UniProt] |
| 预测分子量 | 28 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG43287 anti-AREG / Amphiregulin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43287 anti-AREG / Amphiregulin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43287 anti-AREG / Amphiregulin antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain and Mouse brain lysates stained with ARG43287 anti-AREG / Amphiregulin antibody at 0.5 µg/ml dilution, overnight at 4°C.
