ARG54405
anti-ARC / NOL3 antibody
anti-ARC / NOL3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ARC / NOL3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody recognizes full-length ARC / NOL3 (25 kD). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARC / NOL3 |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 191-208 of human ARC / NOL3. |
| 偶联标记 | Un-conjugated |
| 別名 | Apoptosis repressor with CARD; MYP; Nucleolar protein of 30 kDa; ARC; Myp; Muscle-enriched cytoplasmic protein; Nop30; NOP; FCM; Nucleolar protein 3; NOP30 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa, KB, A549 and Human heart |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NOL3 |
| 全名 | nucleolar protein 3 (apoptosis repressor with CARD domain) |
| 背景介绍 | This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| 生物功能 | Isoform 1: May be involved in RNA splicing. Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors (PubMed:15004034). Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis (PubMed:15509781). Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation (By similarity). Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner (By similarity). Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I (By similarity). Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia (By similarity). Inhibits too myoblast differentiation through caspase inhibition (By similarity). [UniProt] |
| 细胞定位 | Isoform 1: Nucleolus. Note: The SR-rich C-terminus mediates nuclear localization. Isoform 2: Mitochondrion, Cytoplasm, Sarcoplasmic reticulum and Membrane. Note: Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Phosphorylation at Thr-149 is required for its antiapoptotic effect by blocking death-inducing signaling complex death-inducing signaling complex (DISC) activity through the control of interaction with CASP8. Phosphorylation at Thr-149 results in translocation to mitochondria and this translocation enables the binding to CASP8. Dephosphorylated at Thr-149 by calcineurin; doesn't inhibit the association between FADD and CASP8 and the consequent apoptosis. Polyubiquitinated by MDM2; promoting proteasomal-dependent degradation in response to apoptotic stimuli. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG54405 anti-ARC / NOL3 antibody IHC image
Immunohistochemistry: Human heart tissue stained with ARG54405 anti-ARC / NOL3 antibody at 5 μg/ml dilution.
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ARG54405 anti-ARC / NOL3 antibody WB image
Western blot: H: HeLa; K: KB; A: A549. The blots were stained with ARG54405 anti-ARC / NOL3 antibody at 1 μg/ml dilution.
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ARG54405 anti-ARC / NOL3 antibody IHC image
Immunohistochemistry: Skeletal muscle stained with ARG54405 anti-ARC / NOL3 antibody at 5 μg/ml dilution.
.JPG)
