ARG62673
anti-ARAP1 antibody [ARAP1-2]
anti-ARAP1 antibody [ARAP1-2] for ICC/IF,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [ARAP1-2] recognizes ARAP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IP, WB |
| 特异性 | The clone ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ARAP1-2 |
| 同位型 | IgG1 |
| 靶点名称 | ARAP1 |
| 抗原物种 | Human |
| 抗原 | His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1 |
| 偶联标记 | Un-conjugated |
| 別名 | Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1; CENTD2; Cnt-d2; Centaurin-delta-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96P48 Human Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 |
|---|---|
| 基因名称 | ARAP1 |
| 全名 | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 |
| 背景介绍 | ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading. |
| 生物功能 | Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity). [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 162 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG62673 anti-ARAP1 antibody [ARAP1-2] ICC/IF image
Immunofluorescence: ARAP1-transfected NCTC cells treated with TRAIL for 0´, 30´ or 60´ stained with ARG62673 anti-ARAP1 antibody [ARAP1-2] (green)
DR4 was detected by rabbit polyclonal antibody (red).
