ARG55111
anti-APC5 antibody
anti-APC5 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes APC5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | APC5 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (17 aa) within aa. 480-530 of Human Apc5 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Anaphase-promoting complex subunit 5; Cyclosome subunit 5; APC5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Kidney Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ANAPC5 |
| 全名 | anaphase promoting complex subunit 5 |
| 背景介绍 | APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three. |
| 生物功能 | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 85 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG55111 anti-APC5 antibody WB image
Western blot: Human kidney tissue lysate stained with ARG55111 anti-APC5 antibody at (A) 1 and (B) 2 ug/ml dilution.
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ARG55111 anti-APC5 antibody IHC image
Immunohistochemistry: APC5 in Rat kidney tissue stained with ARG55111 anti-APC5 antibody at 20 ug/ml dilution.
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ARG55111 anti-APC5 antibody IHC image
Immunohistochemistry: APC5 in Rat kidney tissue stained with ARG55111 anti-APC5 antibody at 5 ug/ml dilution.
