ARG43628
anti-APC5 antibody
anti-APC5 antibody for Flow cytometry,ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes APC5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | APC5 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment within a.a. 1-300 of Human APC5. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Anaphase Promoting Complex Subunit 5 |
| 別名 | Anaphase-promoting complex subunit 5; Cyclosome subunit 5; APC5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HL60 | ||||||||
| 实际分子量 | 85 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UJX4 Human Anaphase-promoting complex subunit 5 |
|---|---|
| 基因名称 | ANAPC5 |
| 全名 | anaphase promoting complex subunit 5 |
| 背景介绍 | This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008] |
| 生物功能 | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| 细胞定位 | Cytoplasm; Cytoskeleton; Nucleus [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Phosphoprotein |
检测图片 (3) Click the Picture to Zoom In
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ARG43628 anti-APC5 antibody ICC/IF image
Immunofluorescence: A431 cells were stained with ARG43628 anti-APC5 antibody at 1:100 overnight at 4°C.
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ARG43628 anti-APC5 antibody WB image
Western blot: K562, HEK293 and HL60 cell lysates stained with ARG43628 anti-APC5 antibody at 1:1000 dilution.
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ARG43628 anti-APC5 antibody FACS image
Flow Cytometry: 293T cells stained with ARG43628 anti-APC5 antibody (Blue) at 1:100 dilution (1x10^6 cells). Unlabelled sample (Red) was used as a control.
