ARG43701
anti-AP2 gamma antibody
anti-AP2 gamma antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AP2 gamma |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AP2 gamma |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to Human AP2 gamma. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Transcription factor AP-2 gamma |
| 別名 | ERF1; TFAP2G; hAP-2g; Transcription factor ERF-1; Transcription factor AP-2 gamma; AP2-GAMMA; Activating enhancer-binding protein 2 gamma; AP2-gamma |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa | ||||||||
| 实际分子量 | ~50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TFAP2C |
| 全名 | transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) |
| 背景介绍 | The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube. [provided by RefSeq, Jul 2008] |
| 生物功能 | Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. [UniProt] |
| 细胞定位 | Nucleus. |
| 预测分子量 | 49 kDa |
| 翻译后修饰 | Sumoylated on Lys-10; which inhibits transcriptional activity. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG43701 anti-AP2 gamma antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG43701 anti-AP2 gamma antibody at 1:100 dilution. Antigen Retrieval: Heat mediated was performed using sodium citrate buffer pH 6.0.
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ARG43701 anti-AP2 gamma antibody WB image
Western blot: Hela cells stained with ARG43701 anti-AP2 gamma antibody at 1:1000 dilution.
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ARG43701 anti-AP2 gamma antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG43701 anti-AP2 gamma antibody at 1:150 dilution.
