ARG63378
anti-ANILLIN / Scraps antibody
anti-ANILLIN / Scraps antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ANILLIN / Scraps |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Cow |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ANILLIN / Scraps |
| 抗原物种 | Human |
| 抗原 | WQPDACYKPIGKP |
| 偶联标记 | Un-conjugated |
| 別名 | FSGS8; scra; Actin-binding protein anillin; Scraps |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 124 kDa |
| 翻译后修饰 | Phosphorylated during mitosis. Ubiquitinated, and this requires FZR1/CDH1. |
检测图片 (3) Click the Picture to Zoom In
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ARG63378 anti-ANILLIN / Scraps antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63378 anti-ANILLIN / Scraps antibody (green) at 10 µg/ml dilution for 1 hour. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63378 anti-ANILLIN / Scraps antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63378 anti-ANILLIN / Scraps antibody at 10 µg/ml dilution followed by HRP-staining.
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ARG63378 anti-ANILLIN / Scraps antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed MCF7 cells permeabilized with 0.5% Triton. Cells were stained with ARG63378 anti-ANILLIN / Scraps antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
