ARG42626

anti-AMPK beta 1 antibody

anti-AMPK beta 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes AMPK beta 1
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 AMPK beta 1
抗原物种 Human
抗原 Synthetic peptide of Human AMPK beta 1.
偶联标记 Un-conjugated
別名 AMPKb; AMPK; HAMPKb; AMPK subunit beta-1; 5'-AMP-activated protein kinase subunit beta-1

应用说明

应用建议
应用 推荐稀释比
FACS1:20
ICC/IF1:100
IHC-P1:20 - 1:500
IP1:20
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa
实际分子量 ~ 40 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
浓度 Batch dependent
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 19079 Mouse PRKAB1

GeneID: 5564 Human PRKAB1

GeneID: 83803 Rat PRKAB1

基因名称 PRKAB1
全名 protein kinase, AMP-activated, beta 1 non-catalytic subunit
背景介绍 The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]
生物功能 Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). [UniProt]
预测分子量 30 kDa
翻译后修饰 Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG42626 anti-AMPK beta 1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG42626 anti-AMPK beta 1 antibody at 1:1000 dilution.