ARG51172
anti-AMPK alpha 1 / 2 antibody
anti-AMPK alpha 1 / 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
参考文献4
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AMPK alpha 1 / 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AMPK alpha 1 / 2 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2. |
| 偶联标记 | Un-conjugated |
| 別名 | AMPK; Acetyl-CoA carboxylase kinase; ACACA kinase; 5'-AMP-activated protein kinase catalytic subunit alpha-2; EC 2.7.11.31; EC 2.7.11.27; HMGCR kinase; PRKAA; AMPK2; EC 2.7.11.1; AMPK subunit alpha-2; AMPKa2; Hydroxymethylglutaryl-CoA reductase kinase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | PRKAA2 |
|---|---|
| 全名 | protein kinase, AMP-activated, alpha 2 catalytic subunit |
| 背景介绍 | Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. |
| 生物功能 | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30182 AMPK-ACC pathway Antibody Panel Related products: AMPK alpha antibodies; AMPK alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | Ubiquitinated. Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1). |
检测图片 (3) Click the Picture to Zoom In
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ARG51172 anti-AMPK alpha 1/2 antibody WB image
Western blot: 30 µg of A549, HepG2, and Mouse liver cell line lysates stained with ARG51172 anti-AMPK alpha 1/2 antibody at 1:500 dilution.
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ARG51172 anti-AMPK alpha 1/2 antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51172 anti-AMPK alpha 1/2 antibody.
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ARG51172 anti-AMPK alpha 1/2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51172 anti-AMPK alpha 1/2 antibody (left) or the same antibody preincubated with blocking peptide (right).
客户反馈
Good
anti-AMPK alpha 1 / 2 antibody
Application：WB
Sample：A549
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Neuregulin-4 protects cardiomyocytes against high-glucose-induced ferroptosis via the AMPK/NRF2 signalling pathway
WB / Mouse
Expression, clinicopathological significance, and prognostic potential of AMPK, p-AMPK, PGC-1α, and TFAM in astrocytomas
IHC-P / Human
The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth
WB / Mouse
Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome.
WB / Human
