anti-AMPK alpha 1/2 antibody

anti-AMPK alpha 1/2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
publication_link 参考文献2


产品描述 Rabbit Polyclonal antibody recognizes AMPK alpha 1/2
反应物种 Hu, Ms, Rat
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 AMPK alpha 1/2
抗原物种 Human
抗原 Peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.
偶联标记 Un-conjugated
別名 AMPK; Acetyl-CoA carboxylase kinase; ACACA kinase; 5'-AMP-activated protein kinase catalytic subunit alpha-2; EC; EC; HMGCR kinase; PRKAA; AMPK2; EC; AMPK subunit alpha-2; AMPKa2; Hydroxymethylglutaryl-CoA reductase kinase


应用 推荐稀释比
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 108079 Mouse PRKAA2

GeneID: 5563 Human PRKAA2

GeneID: 78975 Rat PRKAA2

基因名称 PRKAA2
全名 protein kinase, AMP-activated, alpha 2 catalytic subunit
背景介绍 Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
生物功能 Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30182 AMPK-ACC pathway Antibody Panel
Related products:
AMPK alpha antibodies; AMPK alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
预测分子量 62 kDa
翻译后修饰 Ubiquitinated.
Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1).

检测图片 (3) Click the Picture to Zoom In

  • ARG51172 anti-AMPK alpha 1/2 antibody WB image

    Western blot: 30 µg of A549, HepG2, and Mouse liver cell line lysates stained with ARG51172 anti-AMPK alpha 1/2 antibody at 1:500 dilution.

  • ARG51172 anti-AMPK alpha 1/2 antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51172 anti-AMPK alpha 1/2 antibody.

  • ARG51172 anti-AMPK alpha 1/2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51172 anti-AMPK alpha 1/2 antibody (left) or the same antibody preincubated with blocking peptide (right).


nuts_pic      Good

anti-AMPK alpha 1/2 antibody



Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC


The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth

WB / Mouse

Jing Liu et al.
BMC Endocr Disord.,  (2022)




Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome.

WB / Human

Zhi Wang et al.
Phytomedicine.,  (2021)