ARG58924
anti-ALT / Alanine Transaminase antibody
anti-ALT / Alanine Transaminase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ALT / Alanine Transaminase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALT / Alanine Transaminase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 118-144 of Human ALT / Alanine Transaminase. |
| 偶联标记 | Un-conjugated |
| 別名 | Glutamate pyruvate transaminase 1; AAT1; GPT1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Alanine aminotransferase 1; ALT1; EC 2.6.1.2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse liver |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GPT |
| 全名 | glutamic-pyruvate transaminase (alanine aminotransferase) |
| 背景介绍 | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| 生物功能 | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 55 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG58924 anti-ALT / Alanine Transaminase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG58924 anti-ALT / Alanine Transaminase antibody at 1:100 dilution.
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ARG58924 anti-ALT / Alanine Transaminase antibody WB image
Western blot: 35 µg of Mouse liver lysate stained with ARG58924 anti-ALT / Alanine Transaminase antibody.
