ARG63274
anti-ALS2CR2 / ILPIP antibody
anti-ALS2CR2 / ILPIP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ALS2CR2 / ILPIP |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALS2CR2 / ILPIP |
| 抗原物种 | Human |
| 抗原 | CDFPDEKDSYWEF |
| 偶联标记 | Un-conjugated |
| 別名 | CALS-21; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein; PAPK; STE20-related kinase adapter protein beta; STRAD beta; ILPIPA; PRO1038; Pseudokinase ALS2CR2; ILPIP; ILP-interacting protein; ALS2CR2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9C0K7 Human STE20-related kinase adapter protein beta |
|---|---|
| 背景介绍 | This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011] |
| 研究领域 | Cancer antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 47 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG63274 anti-ALS2CR2 / ILPIP antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG63274 anti-ALS2CR2 / ILPIP antibody at 1 µg/ml dilution.
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ARG63274 anti-ALS2CR2 / ILPIP antibody WB image
Western Blot: 1). Mock transfection; 2) ILPIP (RC203432) expressing plasmid transfected HEK293 cell lysate standed with ARG63274 anti-ALS2CR2 / ILPIP antibody
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ARG63274 anti-ALS2CR2 / ILPIP antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG63274 anti-ALS2CR2 / ILPIP antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63274 anti-ALS2CR2 / ILPIP antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63274 anti-ALS2CR2 / ILPIP antibody at 3.8 µg/ml dilution followed by AP-staining.
