ARG58177
anti-ALP / Alkaline Phosphatase antibody
anti-ALP / Alkaline Phosphatase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ALP / Alkaline Phosphatase |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALP / Alkaline Phosphatase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 274-304 of Human ALP / Alkaline Phosphatase. |
| 偶联标记 | Un-conjugated |
| 別名 | Intestinal-type alkaline phosphatase; EC 3.1.3.1; IAP; Intestinal alkaline phosphatase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human placenta |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P09923 Human Intestinal-type alkaline phosphatase |
|---|---|
| 基因名称 | ALPI |
| 全名 | alkaline phosphatase, intestinal |
| 背景介绍 | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014] |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| 预测分子量 | 57 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58177 anti-ALP / Alkaline Phosphatase antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were stained with ARG58177 anti-ALP / Alkaline Phosphatase antibody (green) at 1:100 dilution, 2 hours at RT. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).
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ARG58177 anti-ALP / Alkaline Phosphatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG58177 anti-ALP / Alkaline Phosphatase antibody at 1:100 dilution.
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ARG58177 anti-ALP / Alkaline Phosphatase antibody WB image
Western blot: 20 µg of Human placenta lysate stained with ARG58177 anti-ALP / Alkaline Phosphatase antibody at 1:1000 dilution.
