ARG43509
anti-ALKBH5 antibody
anti-ALKBH5 antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ALKBH5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALKBH5 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human ALKBH5. |
| 偶联标记 | Un-conjugated |
| 別名 | OFOXD1; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; Alkylated DNA repair protein alkB homolog 5; ABH5; OFOXD; EC 1.14.11.-; RNA demethylase ALKBH5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | 52 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ALKBH5 |
| 全名 | AlkB family member 5, RNA demethylase |
| 背景介绍 | Enables mRNA N6-methyladenosine dioxygenase activity and molecular condensate scaffold activity. Involved in non-membrane-bounded organelle assembly; post-transcriptional regulation of gene expression; and response to hypoxia. Acts upstream of or within regulation of mRNA export from nucleus and regulation of mRNA processing. Located in Golgi apparatus; cytosol; and nuclear speck. Is active in paraspeckles. [provided by Alliance of Genome Resources, Nov 2024] |
| 生物功能 | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for spermatogenesis (By similarity). [UniProt] |
| 细胞定位 | Nucleus speckle. [UniProt] |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Acetylation; Disulfide bond; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG43509 anti-ALKBH5 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG43509 anti-ALKBH5 antibody at 5 μg/ml dilution.
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ARG43509 anti-ALKBH5 antibody WB image
Western blot: 293T and urkat stained with ARG43509 anti-ALKBH5 antibody at 0.25 μg/ml dilution.
