ARG40178
anti-ALKBH5 antibody
anti-ALKBH5 antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ALKBH5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALKBH5 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 302-330 of Human ALKBH5. |
| 偶联标记 | Un-conjugated |
| 別名 | OFOXD1; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; Alkylated DNA repair protein alkB homolog 5; ABH5; OFOXD; EC 1.14.11.-; RNA demethylase ALKBH5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ALKBH5 |
| 全名 | AlkB family member 5, RNA demethylase |
| 生物功能 | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for spermatogenesis (By similarity). [UniProt] |
| 细胞定位 | Nucleus speckle. [UniProt] |
| 产品亮点 | Related news: m6A reader YTHDF2 in mRNA decay and aggresome formation; |
| 预测分子量 | 44 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG40178 anti-ALKBH5 antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG40178 anti-ALKBH5 antibody.
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ARG40178 anti-ALKBH5 antibody FACS image
Flow Cytometry: U-2 OS cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG40178 anti-ALKBH5 antibody (right histogram) at 1:25 dilution for 60 min at 37ºC, followed by incubation with DyLight® 488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 at 1 μg/10^6 cells used under the same conditions. Acquisition of > 10000 events was performed.
