ARG45174
anti-ALKBH1 antibody
anti-ALKBH1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ALKBH1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALKBH1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to C-terminal region of human ALKBH1. |
| 偶联标记 | Un-conjugated |
| 別名 | ALKBH1; alkB homolog 1, histone H2A dioxygenase; DNA lyase ABH1; EC 4.2.99.18; ABH; Alkylated DNA repair protein alkB homolog 1; DNA oxidative demethylase ALKBH1; alkB; hABH; ABH1; Alpha-ketoglutarate-dependent dioxygenase ABH1; ALKBH; EC 1.14.11.33 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 43 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ALKBH1 |
| 全名 | alkB homolog 1, histone H2A dioxygenase |
| 背景介绍 | This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008] |
| 生物功能 | Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. [UniProt] |
| 细胞定位 | Nucleus; Mitochondrion. [UniProt] |
| 预测分子量 | 44 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG45174 anti-ALKBH1 antibody IHC-P image
Immunohistochemistry: Human intestinal cancer stained with ARG45174 anti-ALKBH1 antibody at 1 μg/ml dilution.
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ARG45174 anti-ALKBH1 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45174 anti-ALKBH1 antibody at 2 ug/ml dilution.
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ARG45174 anti-ALKBH1 antibody WB image
Western blot: Jurkat and K562 stained with ARG45174 anti-ALKBH1 antibody at 0.25 μg/ml dilution.
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ARG45174 anti-ALKBH1 antibody FACS image
Flow Cytometry: A431 stained with ARG45174 anti-ALKBH1 antibody at 1 µg/10^6 cells dilution.
