概述

产品描述	Rabbit Polyclonal antibody recognizes ALIX
反应物种	Hu, Ms, Rat
应用	FACS, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ALIX
抗原物种	Human
抗原	Synthetic peptide derived from Human ALIX.
偶联标记	Un-conjugated
別名	Programmed cell death 6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; DRIP4; PDCD6-interacting protein; Hp95; ALIX; HP95; AIP1

应用说明

应用建议	应用 推荐稀释比 FACS 1:100 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量	96 kDa

属性

形式	Liquid
纯化	Affinity purified
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 10015 Human PDCD6IP GeneID: 18571 Mouse PDCD6IP GeneID: 501083 Rat PDCD6IP Swiss-port # Q8WUM4 Human Programmed cell death 6-interacting protein Swiss-port # Q9QZA2 Rat Programmed cell death 6-interacting protein Swiss-port # Q9WU78 Mouse Programmed cell death 6-interacting protein More
基因名称	PDCD6IP
全名	programmed cell death 6 interacting protein
背景介绍	This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]
生物功能	Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. [UniProt]
细胞定位	Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Secreted, exosome. Cell junction, tight junction. Midbody, Midbody ring. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 at centrosomes of non-dividing cells. Component of the actomyosin-tight junction complex (By similarity). PDCD6IP targeting to the midbody requires the interaction with CEP55 (PubMed:18641129). [UniProt]
预测分子量	96 kDa
翻译后修饰	May be phosphorylated on tyrosine residues by activated PDGFRB. [UniProt]

检测图片 (1) Click the Picture to Zoom In