ARG59775
anti-ALDH4A1 antibody
anti-ALDH4A1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ALDH4A1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ALDH4A1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 354-563 of Human ALDH4A1 (NP_003739.2). |
| 偶联标记 | Un-conjugated |
| 別名 | P5CD; ALDH4; P5CDh; Aldehyde dehydrogenase family 4 member A1; L-glutamate gamma-semialdehyde dehydrogenase; Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial; P5C dehydrogenase; EC 1.2.1.88 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse skeletal muscle and BT474 | ||||||
| 实际分子量 | 65 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30038 Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Swiss-port # Q8CHT0 Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial |
|---|---|
| 基因名称 | ALDH4A1 |
| 全名 | aldehyde dehydrogenase 4 family, member A1 |
| 背景介绍 | This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009] |
| 生物功能 | Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes. [UniProt] |
| 细胞定位 | Mitochondrion matrix. [UniProt] |
| 预测分子量 | 62 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG59775 anti-ALDH4A1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG59775 anti-ALDH4A1 antibody.
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ARG59775 anti-ALDH4A1 antibody WB image
Western blot: 25 µg of Mouse skeletal muscle and BT474 cell lysates stained with ARG59775 anti-ALDH4A1 antibody at 1:1000 dilution.
