ARG56997
anti-AKR1C1 antibody [6D10]
anti-AKR1C1 antibody [6D10] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [6D10] recognizes AKR1C1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6D10 |
| 同位型 | IgG1, kappa |
| 靶点名称 | AKR1C1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-323 of Human AKR1C1. |
| 偶联标记 | Un-conjugated |
| 別名 | 20-alpha-hydroxysteroid dehydrogenase; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; EC 1.1.1.112; EC 1.1.1.-; 20-alpha-HSD; Chlordecone reductase homolog HAKRC; DD1; HBAB; C9; DD1/DD2; High-affinity hepatic bile acid-binding protein; Indanol dehydrogenase; 20-ALPHA-HSD; Aldo-keto reductase family 1 member C1; DDH1; EC 1.1.1.149; H-37; MBAB; EC 1.3.1.20; DDH; Dihydrodiol dehydrogenase 1/2; HAKRC; 2-ALPHA-HSD |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q04828 Human Aldo-keto reductase family 1 member C1 |
|---|---|
| 基因名称 | AKR1C1 |
| 全名 | aldo-keto reductase family 1, member C1 |
| 背景介绍 | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008] |
| 生物功能 | Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. [UniProt] |
| 预测分子量 | 37 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG56997 anti-AKR1C1 antibody [6D10] FACS image
Flow Cytometry: A431 cell line stained with ARG56997 anti-AKR1C1 antibody [6D10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56997 anti-AKR1C1 antibody [6D10] WB image
Western blot: 1) 50 ng of Recombinant Human AKR1C1, 2) 40 µg of HepG2 cell lysate, and 3) 40 µg of Raji cell lysate stained with ARG56997 anti-AKR1C1 antibody [6D10] at 1:1000.
