ARG45799

anti-AKAP8 / AKAP95 antibody

anti-AKAP8 / AKAP95 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes AKAP8 / AKAP95
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 AKAP8 / AKAP95
抗原物种 Human
抗原 Recombinant protein containing to human AKAP8 / AKAP95 .
偶联标记 Un-conjugated
別名 AKAP8; A-Kinase Anchoring Protein 8; AKAP-8; AKAP95; AKAP 95; A-kinase anchor protein 95 kDa; AKAP-95; A-kinase anchor protein 8

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 92 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10270 Human AKAP8

Swiss-port # O43823 Human A-kinase anchor protein 8

基因名称 AKAP8
全名 A-Kinase Anchoring Protein 8
背景介绍 This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
生物功能 Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). [UniProt]
细胞定位 Cytoplasm; Nucleus. [UniProt]
预测分子量 76 kDa
翻译后修饰 Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45799 anti-AKAP8 / AKAP95 antibody IHC-P image

    Immunohistochemistry: Human appendiceal adenocarcinoma stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 2 μg/ml dilution.

  • ARG45799 anti-AKAP8 / AKAP95 antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 5 μg/ml dilution.

  • ARG45799 anti-AKAP8 / AKAP95 antibody WB image

    Western blot: HEK293 stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 0.5 μg/ml dilution.

  • ARG45799 anti-AKAP8 / AKAP95 antibody FACS image

    Flow Cytometry: A431 stained with ARG45799 anti-AKAP8 / AKAP95 antibody at 1 µg/10^6 cells dilution.