ARG56921

anti-AK3 antibody [SJB3-36]

anti-AK3 antibody [SJB3-36] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Mouse Monoclonal antibody [SJB3-36] recognizes AK3
反应物种 Hu, Ms
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 SJB3-36
同位型 IgG1, kappa
靶点名称 AK3
抗原物种 Human
抗原 Recombinant fragment of Human AK3.
偶联标记 Un-conjugated
別名 EC 2.7.4.10; AKL3L; Adenylate kinase 3; Adenylate kinase 3 alpha-like 1; FIX; AK6; AK3L1; AKL3L1; GTP:AMP phosphotransferase AK3, mitochondrial; AK 3

应用说明

应用建议
应用 推荐稀释比
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 10% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 50808 Human AK3

GeneID: 56248 Mouse AK3

Swiss-port # Q9UIJ7 Human GTP:AMP phosphotransferase AK3, mitochondrial

Swiss-port # Q9WTP7 Mouse GTP:AMP phosphotransferase AK3, mitochondrial

基因名称 AK3
全名 adenylate kinase 3
背景介绍 The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
生物功能 Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. [UniProt]
预测分子量 26 kDa

检测图片 (6) Click the Picture to Zoom In

  • ARG56921 anti-AK3 antibody [SJB3-36] ICC/IF image

    Immunoflorescense: U87MG cell line stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG56921 anti-AK3 antibody [SJB3-36] FACS image

    Flow Cytometry: Jurkat cell line stained with ARG56921 anti-AK3 antibody [SJB3-36] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG56921 anti-AK3 antibody [SJB3-36] IHC-P image

    Immunohistochemistry: Paraffin-embedded sections of Human liver tissue stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:100 for 2 hours at RT.

  • ARG56921 anti-AK3 antibody [SJB3-36] WB image

    Western blot: 50 ng of 1) Recombinant Human AK1, 2) Recombinant Human AK2, 3) Recombinant Human AK3 stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:1000. .

  • ARG56921 anti-AK3 antibody [SJB3-36] WB image

    Western blot: 40 µg of 1) Hep3B cell lysate, 2) 293T cell lysate, 3) Mouse kidney tissue lysate stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:1000.

  • ARG56921 anti-AK3 antibody [SJB3-36] WB image

    Western blot: 40 µg of Mouse liver tissue lysate stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:1000.