ARG57175
anti-AK1 antibody [7E9]
anti-AK1 antibody [7E9] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [7E9] recognizes AK1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 7E9 |
| 同位型 | IgG1, kappa |
| 靶点名称 | AK1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-194 of Human AK1 |
| 偶联标记 | Un-conjugated |
| 別名 | Myokinase; Adenylate kinase isoenzyme 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; EC 2.7.4.6; HTL-S-58j; EC 2.7.4.3; Adenylate monophosphate kinase; AK 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AK1 |
| 全名 | adenylate kinase 1 |
| 背景介绍 | Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. [UniProt] |
| 预测分子量 | 22 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG57175 anti-AK1 antibody [7E9] WB image
Western blot: 40 µg of 1) HeLa, 2) U87-MG, 3) K562, 4) 293T, 5) HepG2, 6) A549, 7) MCF7, 8) SK-OV-3, and 9) PC3 cell lysates stained with ARG57175 anti-AK1 antibody [7E9] at 1:1000.
