ARG42926
anti-AIMP2 antibody
anti-AIMP2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AIMP2 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AIMP2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 298-320 of Human AIMP2. (NVQRWMRSCENLAPFNTALKLLK) |
| 偶联标记 | Un-conjugated |
| 別名 | PRO0992; JTV-1; JTV1; P38; Protein JTV-1; Multisynthase complex auxiliary component p38; Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 36 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13155 Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Swiss-port # Q32PX2 Rat Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 |
|---|---|
| 基因名称 | AIMP2 |
| 全名 | aminoacyl tRNA synthetase complex-interacting multifunctional protein 2 |
| 背景介绍 | The protein encoded by this gene is part of the aminoacyl-tRNA synthetase complex, which contains nine different aminoacyl-tRNA synthetases and three non-enzymatic factors. The encoded protein is one of the non-enzymatic factors and is required for assembly and stability of the complex. [provided by RefSeq, May 2016] |
| 生物功能 | Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed:19131329). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Nucleus. Note=Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus. [UniProt] |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Phosphorylated on serine residues in response to UV irradiation. Ubiquitinated by PRKN, leading to its degradation by the proteasome. Mutant PRKN fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG42926 anti-AIMP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42926 anti-AIMP2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42926 anti-AIMP2 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat liver, Rat lung, Rat kidney, Rat brain, Jurkat, CEM, HUT and U93T cell lysates stained with ARG42926 anti-AIMP2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42926 anti-AIMP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42926 anti-AIMP2 antibody at 1 µg/ml dilution, overnight at 4°C.
